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Anti-TCF4/TCF7L2 antibody produced in rabbit

SIGMA/SAB5701262

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SAB5701262-100UL
$405.00
1/EA		 Add To Favorites
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung cancer using TCF4/TCF4/TCF7L2 antibody (SAB5701262) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using TCF4/TCF4/TCF7L2 antibody (SAB5701262) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using TCF4/TCF4/TCF7L2 antibody (SAB5701262) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using TCF4/TCF4/TCF7L2 antibody (SAB5701262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Chromatin Immunoprecipitation Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using TCF4/TCF7L2 antibody (SAB5701262) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

 

antibody product type primary antibodies
biological source rabbit
clone monoclonal
concentration 0.13 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity human, mouse, rat
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. Q9NQB0 
General description: This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]
Immunogen: A synthesized peptide derived from human TCF4/TCF4/TCF7L2 .
Physical form: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage Temp. −20°C

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