Anti- NF-kB p65/RelA antibody produced in rabbit
SIGMA/SAB5701321
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody (SAB5701321) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody (SAB5701321) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (SAB5701321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
Western Blotting Western blot analysis of extracts from normal (control) and NF-kB p65/RelA knockout (KO) HeLa cells, using NF-kB p65/RelA antibody (SAB5701321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
Western Blotting Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (SAB5701321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 3 min.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg NF-kB p65/RelA antibody (SAB5701321). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (SAB5701321) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.25 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:1000-1:3000 | |
| UniProt accession no. | Q04206  |
| General description: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen: | A synthesized peptide derived from human NF-κB p65. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |