Anti- β-Catenin antibody produced in rabbit
SIGMA/SAB5701324
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] β-Catenin Rabbit mAb (SAB5701324) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] β-Catenin Rabbit mAb (SAB5701324) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] β-Catenin Rabbit mAb (SAB5701324) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] β-Catenin Rabbit mAb (SAB5701324) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using β-Catenin antibody (SAB5701324) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HeLa cells, using β-Catenin antibody (SAB5701324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (SAB5701324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of Mouse brain, using β-Catenin antibody (SAB5701324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg β-Catenin antibody (SAB5701324). Western blot was performed from the immunoprecipitate using β-Catenin (SAB5701324) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.427 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P35222  |
| General description: | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
| Immunogen: | A synthesized peptide derived from human beta Catenin. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |