Anti-Bcl-2 antibody produced in rabbit
SIGMA/SAB5701336
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using Bcl-2 antibody (SAB5701336) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (SAB5701336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of Mouse lung using 3 μg Bcl-2 antibody (SAB5701336). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (SAB5701336) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.48 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P10415  |
| General description: | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Immunogen: | A synthesized peptide derived from human Bcl-2. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |