Anti-VDAC1 antibody produced in rabbit
SIGMA/SAB5701340
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon carcinoma using VDAC1 antibody (SAB5701340) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of L929 cells using VDAC1 antibody (SAB5701340). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using VDAC1 antibody (SAB5701340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.21 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P21796  |
| General description: | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
| Immunogen: | A synthesized peptide derived from human VDAC1. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |