Anti-IL2 antibody produced in rabbit
SIGMA/SAB5701391
Product Type: Chemical
Western Blotting Western blot analysis of extracts of 293T cells, using IL2 antibody (SAB5701391) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of Jurkat cells, using IL2 antibody (SAB5701391) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM),A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Flow Cytometry Flow cytometry was performed with Jurkat cells.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1.51 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P60568  |
| General description: | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
| Immunogen: | Recombinant protein of human IL2. |
| Physical form: | Buffer: PBS with 0.02% Thimerosal,pH7.3. |
| Storage Temp. | 2-8°C |