Anti- Bax antibody produced in rabbit
SIGMA/SAB5701392
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat lymph node using Bax Rabbit mAb (SAB5701392) at dilution of 1:25, 1:50 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon carcinoma using Bax Rabbit mAb (SAB5701392) at dilution of 1:25, 1:50 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spleen using Bax Rabbit mAb (SAB5701392) at dilution of 1:25, 1:50 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using Bax Rabbit mAb (SAB5701392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Bax Rabbit mAb (SAB5701392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using Bax Rabbit mAb (SAB5701392) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HT-1080 cells, using Bax antibody (SAB5701392) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
Western Blotting Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (SAB5701392) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Bax antibody (SAB5701392). Western blot was performed from the immunoprecipitate using Bax antibody (SAB5701392) at a dilition of 1:500.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q07812  |
| General description: | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. |
| Immunogen: | A synthetic peptide of human Bax. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |