Anti-Ceruloplasmin antibody produced in rabbit
SIGMA/SAB5701394
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat liver using Ceruloplasmin Rabbit mAb (SAB5701394) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver using Ceruloplasmin Rabbit mAb (SAB5701394) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of rat liver using Ceruloplasmin Rabbit mAb (SAB5701394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of human liver using Ceruloplasmin Rabbit mAb (SAB5701394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of mouse liver using Ceruloplasmin Rabbit mAb (SAB5701394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Ceruloplasmin antibody (SAB5701394) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P00450  |
| General description: | The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. |
| Immunogen: | A synthetic peptide of human Ceruloplasmin. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |