Anti- NT5E / CD73 antibody produced in rabbit
SIGMA/SAB5701396
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using NT5E/CD73 antibody (SAB5701396) at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat spleen using NT5E/CD73 antibody (SAB5701396) at dilution of 1:200 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using NT5E / CD73 antibody (SAB5701396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts from normal (control) and NT5E / CD73 Rabbit pAb knockout (KO) HCT116 cells, using NT5E / CD73 Rabbit pAb antibody (SAB5701396) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of Rat eye, using NT5E / CD73 antibody (SAB5701396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.05 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P21589  |
| General description: | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |