Anti- BRG1/SMARCA4 antibody produced in rabbit
SIGMA/SAB5701401
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] BRG1/BRG1/SMARCA4 Rabbit pAb (SAB5701401) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] BRG1/BRG1/SMARCA4 Rabbit pAb (SAB5701401) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of A549 cells using BRG1/BRG1/SMARCA4 antibody (SAB5701401). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of 293T cells, using BRG1/BRG1/SMARCA4 antibody (SAB5701401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 1 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg BRG1/BRG1/SMARCA4 antibody (SAB5701401). Western blot was performed from the immunoprecipitate using BRG1/BRG1/SMARCA4 antibody (SAB5701401) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.25 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P51532  |
| General description: | The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 30-130 of human BRG1/BRG1/SMARCA4 (NP_003063.2). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |