Anti- ATF2 antibody produced in rabbit
SIGMA/SAB5701402
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using ATF2 Rabbit pAb (SAB5701402) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spinal cord using ATF2 Rabbit pAb (SAB5701402) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using ATF2 antibody (SAB5701402). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using ATF2 antibody (SAB5701402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western Blotting Western blot analysis of extracts from normal (control) and ATF2 Rabbit pAb knockout (KO) 293T cells, using ATF2 Rabbit pAb antibody (SAB5701402) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.18 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P15336  |
| General description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell′s DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ATF2 (NP_001871.2). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |