Anti-DNA Polymerase beta antibody produced in rabbit
SIGMA/SAB5701419
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using DNA Polymerase beta Rabbit mAb (SAB5701419) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using DNA Polymerase beta Rabbit mAb (SAB5701419) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using DNA Polymerase beta Rabbit mAb (SAB5701419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.33 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P06746  |
| General description: | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| Immunogen: | A synthesized peptide derived from human DNA Polymerase beta |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |