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Anti-ALOX5 antibody produced in rabbit

SIGMA/SAB5701424

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SAB5701424-100UL
$405.00
1/EA		 Add To Favorites
Immunohistochemistry Immunohistochemistry of paraffin-embedded human appendix using ALOX5 Rabbit mAb (SAB5701424) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spleen using ALOX5 Rabbit mAb (SAB5701424) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using ALOX5 Rabbit mAb (SAB5701424) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of Raji cells, using ALOX5 Rabbit mAb (SAB5701424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Western Blotting Western blot analysis of extracts of Rat lung, using ALOX5 Rabbit mAb (SAB5701424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.

 

antibody product type primary antibodies
biological source rabbit
clone monoclonal
concentration 1.45 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity human, mouse, rat
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. P09917 
General description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Immunogen: A synthesized peptide derived from human ALOX5
Physical form: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage Temp. −20°C

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