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Anti-MonoMethyl-Histone H4-R3 antibody produced in rabbit

SIGMA/SAB5701428

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SAB5701428-100UL
$405.00
1/EA		 Add To Favorites
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using MonoMethyl-Histone H4-R3 antibody (SAB5701428) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H4-R3 antibody (SAB5701428) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using MonoMethyl-Histone H4-R3 antibody (SAB5701428) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 antibody (SAB5701428). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-R3 antibody (SAB5701428). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

 

antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 4.06 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity mouse, human, rat
storage temp. −20°C
technique(s) western blot: 1:500-1:2000
UniProt accession no. P62805 
General description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Immunogen: A synthetic peptide of human MonoMethyl-Histone H4-R3
Physical form: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Temp. −20°C

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