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Anti-MonoMethyl-Histone H3-R26 antibody produced in rabbit

SIGMA/SAB5701429

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SAB5701429-100UL
$405.00
1/EA		 Add To Favorites
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using H3R26me1 antibody (SAB5701429) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-R26 antibody (SAB5701429). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-R26 antibody (SAB5701429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot Blot Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R26 antibody (SAB5701429).

 

antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 0.44 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity rat, human, mouse
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. Q16695 
General description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunogen: A synthetic methylated peptide corresponding to residues surrounding R26 of human histone H3
Physical form: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Temp. −20°C

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