Anti-YB-1/YBX1 antibody produced in rabbit
SIGMA/SAB5701462
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human oophoroma using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using YB-1/YBX1 Rabbit mAb (SAB5701462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HeLa cells, using YB-1/YBX1 Rabbit mAb (SAB5701462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts of various cell lines, using YB-1/YBX1 Rabbit mAb (SAB5701462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg YB-1/YBX1 antibody (SAB5701462). Western blot was performed from the immunoprecipitate using YB-1/YBX1 (SAB5701462) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.4 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| UniProt accession no. | P67809  |
| General description: | This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015] |
| Immunogen: | A synthesized peptide derived from human YB-1/YBX1 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |