Anti-SMC1 antibody produced in rabbit
SIGMA/SAB5701554
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of C6 cells using SMC1 Rabbit mAb (SAB5701554) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using SMC1 Rabbit mAb (SAB5701554) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using SMC1 Rabbit mAb (SAB5701554) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using SMC1 Rabbit mAb (SAB5701554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 s.
Western Blotting Western blot analysis of extracts of various cell lines, using SMC1 Rabbit mAb (SAB5701554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.21 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q14683  |
| General description: | Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| Immunogen: | A synthesized peptide derived from human SMC1 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |