Anti- L3MBTL3 antibody produced in rabbit
SIGMA/SAB5701640
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (SAB5701640). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of 293T cells, using L3MBTL3 antibody (SAB5701640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg L3MBTL3 antibody (SAB5701640). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (SAB5701640) at a dilition of 1:1000.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.11 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q96JM7  |
| General description: | This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma. [provided by RefSeq, Oct 2016] |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |