Anti-Acetyl-Histone H4-K91 antibody produced in rabbit
SIGMA/SAB5701642
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K91 antibody (SAB5701642) at dilution of 1:100.C6 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K91 antibody (SAB5701642) at dilution of 1:100.HeLa cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 antibody (SAB5701642) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K91 antibody (SAB5701642) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 300s.
Chromatin Immunoprecipitation Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K91 antibody (SAB5701642) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.29 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P62805  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
| Immunogen: | A synthetic Peptide of human Acetyl-Histone H4-K91 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |