Anti- B4GALT1 antibody produced in rabbit

SIGMA/SAB5701649

Product Type: Chemical

Catalog Number	Price


45-SAB5701649-100UL	$371.00 1/EA	Add To Favorites

Western Blotting Western blot analysis of extracts of various cell lines, using B4GALT1 antibody (SAB5701649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 90 s.

Western Blotting Western blot analysis of extracts from normal (control) and B4GALT1 knockout (KO) HeLa cells, using B4GALT1 antibody (SAB5701649) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.

Properties
Descriptions
Basic Data

antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	1.23 mg/mL
form	liquid
Quality Level	100
shipped in	wet ice
species reactivity	mouse, rat, human
storage temp.	−20°C
technique(s)	immunohistochemistry: 1:50-1:200
	western blot: 1:500-1:2000
UniProt accession no.	P15291

General description:	This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5′ end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-215 of human B4GALT1 (NP_001488.2).
Physical form:	Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Storage Temp.

−20°C

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