Anti-Xanthine Oxidase (XDH) antibody produced in rabbit
SIGMA/SAB5701666
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using Xanthine Oxidase (XDH) (XDH) Rabbit mAb (SAB5701666) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human oophoroma using Xanthine Oxidase (XDH) (XDH) Rabbit mAb (SAB5701666) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using Xanthine Oxidase (XDH) (XDH) Rabbit mAb (SAB5701666) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Xanthine Oxidase (XDH) (XDH) Rabbit mAb (SAB5701666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Xanthine Oxidase (XDH) (XDH) Rabbit mAb (SAB5701666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.37 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P47989  |
| General description: | Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014] |
| Immunogen: | A synthesized peptide derived from human Xanthine Oxidase (XDH) (XDH) |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |