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Anti-TARBP2 antibody produced in rabbit

SIGMA/SAB5701675

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5701675-100UL
$405.00
1/EA
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Immunohistochemistry Immunohistochemistry of paraffin-embedded rat pancreas using TARBP2 Rabbit mAb (SAB5701675) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using TARBP2 Rabbit mAb (SAB5701675) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse pancreas using TARBP2 Rabbit mAb (SAB5701675) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using TARBP2 Rabbit mAb (SAB5701675) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using TARBP2 Rabbit mAb (SAB5701675) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using TARBP2 Rabbit mAb (SAB5701675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 min.

 

antibody product type primary antibodies
biological source rabbit
clone monoclonal
concentration 0.55 mg/mL
form liquid
Quality Level 100 
shipped in wet ice
species reactivity mouse, human, rat
storage temp. −20°C
technique(s) immunohistochemistry: 1:50-1:200
  western blot: 1:500-1:2000
UniProt accession no. Q15633 
General description: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]
Immunogen: A synthesized peptide derived from human TARBP2
Physical form: Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage Temp. −20°C

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