Anti-Carbonic Anhydrase 1 (CA2) antibody produced in rabbit
SIGMA/SAB5701682
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using Carbonic Anhydrase 1 (CA2) Rabbit mAb (SAB5701682) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using Carbonic Anhydrase 1 (CA2) Rabbit mAb (SAB5701682) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using Carbonic Anhydrase 1 (CA2) Rabbit mAb (SAB5701682) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 1 (CA2) Rabbit mAb (SAB5701682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.25 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P00918  |
| General description: | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| Immunogen: | A synthesized peptide derived from human Carbonic Anhydrase 2 (CA2) |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |