Anti-CARD11 antibody produced in rabbit
SIGMA/SAB5701708
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat spleen using CARD11 Rabbit mAb (SAB5701708) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse spleen using CARD11 Rabbit mAb (SAB5701708) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of rat spleen using CARD11 Rabbit mAb (SAB5701708) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of mouse spleen using CARD11 Rabbit mAb (SAB5701708) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using CARD11 Rabbit mAb (SAB5701708) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1.92 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q9BXL7  |
| General description: | The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human CARD11 |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |