Anti-Phospho-Histone H2AX-S139 antibody produced in rabbit
SIGMA/SAB5701787
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody (SAB5701787) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (SAB5701787) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (SAB5701787) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using Phospho-Histone H2AX-S139 antibody (SAB5701787). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using Phospho-Histone H2AX-S139 antibody (SAB5701787). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H2AX-S139 antibody (SAB5701787). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (SAB5701787) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.33 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P16104  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Immunogen: | A synthesized peptide derived from human Phospho-Histone H2A.X (S139). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |