Anti-Phospho-Smad3-S423/S425 antibody produced in rabbit
SIGMA/SAB5701792
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human esophageal using Phospho-Smad3-S423/S425 antibody (SAB5701792) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Phospho-Smad3-S423/S425 antibody (SAB5701792) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Smad3-S423/S425 antibody (SAB5701792) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (SAB5701792) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.5 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P84022  |
| General description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009] |
| Immunogen: | A synthesized peptide derived from human Phospho-Smad3 (S423 + S425). |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |