Anti-Phospho-MYL9-T18/S19 antibody produced in rabbit
SIGMA/SAB5701816
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded Rat ovary using Phospho-MYL9-T18/S19 antibody (SAB5701816) at dilution of 1:100 (20x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human gastric cancer using Phospho-MYL9-T18/S19 antibody (SAB5701816) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-MYL9-T18/S19 antibody (SAB5701816) at 1:1000 dilution.293T cells and C2C12 and C6 cells were treated by ATP(5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.65 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P24844  |
| General description: | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around of human Phospho-MYL9-T18/S19 . |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |