Anti-Phospho-POLR2A-S7 antibody produced in rabbit
SIGMA/SAB5701818
Product Type: Chemical
Western Blotting Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A-S7 antibody (SAB5701818) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 μM) at 37 °C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.68 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P24928  |
| General description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. |
| Immunogen: | A synthetic phosphorylated peptide around of human Phospho-POLR2A-S7. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |