Anti-Phospho-α-Synuclein-Y133 antibody produced in rabbit
SIGMA/SAB5701880
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-α-Synuclein-Y133 pAb (SAB5701880) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 60 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.51 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P37840  |
| General description: | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer′s disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y133 of human alpha-Synuclein. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |