Anti-Phospho-EZR-T567/RDX-T564/MSN-T558 antibody produced in rabbit
SIGMA/SAB5701897
Product Type: Chemical
Western Blotting Western blot analysis of extracts of PC-12 cells, using Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb (SAB5701897) at 1:1000 dilution.PC-12 cells were treated by ATP(5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.06 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| General description: | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T567/T564/T558 of human EZR/RDX/MSN. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |