Anti-Pan Phospho-Tyrosine antibody produced in rabbit
SIGMA/SAB5701920
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using Pan Phospho-Tyrosine antibody (SAB5701920) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 1.5 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | ?  |
| Immunogen: | A synthetic peptide corresponding to a sequence containing phosphorylated Y. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |