Anti-Phospho-KRAS-S89 antibody produced in rabbit
SIGMA/SAB5701925
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Phospho-KRAS-S89 Rabbit pAb (SAB5701925) at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-KRAS-S89 Rabbit pAb (SAB5701925) at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-KRAS-S89 Rabbit pAb (SAB5701925) at 1:500 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes.NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 180 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.647 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P01116  |
| General description: | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S89 of human KRAS. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |