Anti-Phospho-AMPKa1/AMPKa2-T183/T172 antibody produced in rabbit
SIGMA/SAB5701926
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1/AMPKa2-T183/T172 antibody (SAB5701926) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 μM) at 37 °C for 30 minutes.C2C12 cells were treated by oligomycin (0.5 μM) at 37 °C for 30 minutes.C6 cells were treated by oligomycin (0.5 μM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 60 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.2 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q13131/P54646  |
| General description: | AMPK belongs to the ser/thr protein kinase family. Alpha subunit is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T183/T172 of human AMPKa1/AMPKa2. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |