Anti-Phospho-CGAS-S291 antibody produced in rabbit
SIGMA/SAB5701930
Product Type: Chemical
Western Blotting Western blot analysis of extracts of 293T cells, using Phospho-CGAS-S291 antibody (SAB5701930) at 1:500 dilution.293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 180 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.439 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q8C6L5  |
| General description: | The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S291 of Mouse CGAS. |
| Physical form: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |