Alanine Racemase from Bacillus stearothermophilus
SIGMA/SAE0023 - buffered aqueous solution, ≥10 IU/mg solid
Synonym: Alanine Racemase from Bacillus stearothermophilus
CAS Number: 9024-06-0
MDL Number: MFCD03454300
Product Type: Chemical
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| biological source | Bacillus sp. (B. stearothermophilus) |
| form | buffered aqueous solution |
| mol wt | Mr 78 kDa (2 subunits 39 kDa each) |
| Quality Level | 200  |
| specific activity | ≥10 IU/mg solid |
| storage temp. | −20°C |
| Application: | Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †. |
| Biochem/physiol Actions: | Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.† |
| Other Notes: | One unit will convert 1.0 μmole of |
| Physical form: | Aqueous solution containing 10 mM potassium phosphate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 IU/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 5.1.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |