Choline Oxidase from Arthrobacter sp.
SIGMA/SAE0044 - recombinant, expressed in E. coli ≥12 units/mg protein
Synonym: COD; COX; ChOx; Choline: oxygen 1-oxidoreductase
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
pH Dependence: Activity is shown as percent of control.
Short time thermal stability: Reconstituted enzyme was incubated for 15 minutes at indicated temperatures and then assayed under standard conditions. Activity is shown as percent of control.
Extended stability study of reconstituted Choline oxidase at 37 °C. Activity is shown as percent of control of a week period.
| biological source | bacterial (arthrobacter sp.) |
| form | powder |
| Quality Level | 200  |
| recombinant | expressed in E. coli ≥12 units/mg protein |
| specific activity | ≥12 units/mg protein |
| storage temp. | −20°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | A0A0Q9NRQ3  |
| A0A0Q9PH20 |
| Application: | Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma. |
| Biochem/physiol Actions: | Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants. |
| General description: | Research area: Cell Signaling Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme. |
| Other Notes: | One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline. |
| Preparation Note: | It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks. |
| Preparation Note: | Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 - H412 |
| Precautionary statements | P261 - P273 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥12 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.17 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |