HRV-3C Protease.
SIGMA/SAE0045 - N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
Synonym: Human Rhinovirus 3C Protease; Levlfqgp site protease; PreScission Protease; Human Rhinovirus 3C Protease; Levlfqgp site protease; PreScission Protease
Product Type: Chemical
SDS-PAGE HRV-3C Protease (Cat. No. SAE0045) was assessed on SDS-PAGE.
| application(s) | life science and biopharma |
| assay | ≥90% (SDS-PAGE) |
| biological source | human ( |
| concentration | 0.8-1.2 mg/mL |
| form | aqueous solution |
| mol wt | 21 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥5000 U/mg |
| storage temp. | −20°C |
| suitability | suitable for protein modification |
| technique(s) | protein purification: suitable |
| Application: | HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein. |
| General description: | HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence. Proteolytic cleavage occurs between the Gln and Gly residues. HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest. This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography. |
| Other Notes: | One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pN |
| Physical form: | Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥5000 U/mg |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |