Carboxypeptidase A from bovine pancreas
SIGMA/SAE0046 - (Type II-PMSF treated), ≥20 units/mg protein
Synonym: Carboxypeptidase A; Carboxypolypeptidase; Peptidyl-L-amino-acid hydrolase
CAS Number: 11075-17-5
MDL Number: MFCD00130718
Product Type: Chemical
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| biological source | bovine pancreas |
| form | aqueous suspension |
| impurities | ≤0.05 BTEE units/mg protein chymotrpsin |
| ≤10 BAEE units/mg protein trypsin | |
| mol wt | ~35 kDa |
| purified by | 2× crystallization |
| quality | (Type II-PMSF treated) |
| Quality Level | 200  |
| specific activity | ≥20 units/mg protein |
| storage temp. | 2-8°C |
| Analysis Note: | Protein determined by E |
| Application: | Carboxypeptidase A from bovine pancreas has been used in in vitro simulated digestion. |
| Application: | Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase. |
| Biochem/physiol Actions: | Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.† |
| General description: | Carboxypeptidase A (CPA) is a secreted protease is liberated after the activation of mast cells to facilitate acute anaphylaxis. Carboxypeptidase A has a long half-life in vivo, when compared to other secreted proteases. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of hippuryl- |
| Packaging: | 500, 2500, 5000 units in glass bottle |
| Preparation Note: | Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.17.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |