L-Lactate Dehydrogenase (LDHA)

SIGMA/SAE0049 - from human, recombinant, expressed in E. coli, aqueous solution

Synonym: Lactic Dehydrogenase, recombinant from E. coli; α-HBDH; α-hydroxy butyratede hydrogenase; anaerobic lactate dehydrogenase; (S)-Lactate: NAD⁺ oxidoreductase; L-Lactate Dehydrogenase; Lactate

CAS Number: 9001-60-9
MDL Number: MFCD00131466
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-SAE0049-10KU	10 kU	$456.00 1/EA	Add To Favorites

SDS-PAGE L-Lactate Dehydrogenase (LDHA) (Cat. No. SAE0049) was assessed on SDS-PAGE.

Properties
Descriptions
Safety Info.
Basic Data

biological source	human
color	colorless
form	aqueous solution
Quality Level	200
recombinant	expressed in E. coli
shipped in	dry ice
storage condition	(Keep container tightly closed in a dry and well-ventilated place)
storage temp.	−20°C
UniProt accession no.	K1T0A2

Application:	L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.
Biochem/physiol Actions:	L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.
Biochem/physiol Actions:	L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.
General description:	Research area: Cell Signaling The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.
Other Notes:	One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
Physical form:	Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.1.1.27 ( BRENDA \| IUBMB )
UNSPSC	12352200

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