Chitotriosidase human
SIGMA/SAE0052 - recombinant, expressed in HEK 293 cells
Synonym: Chitinase-1; Chitotriosidase-1
Product Type: Chemical
Unit Definition One unit is defined as the amount of CT required to produce 1 pmol of 4-Methylumbelliferone from 4-Methylumbelliferyl beta -D-
N′,N”-triacetylchitotrioside per minute at 25 ℃, pH 5.6.
N′,N”-triacetylchitotrioside per minute at 25 ℃, pH 5.6.
Purity
The purity of human, recombinant Chitotriosidase expressed in HEK 293 cells (Cat. No. SAE0052) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L).
(Representative result)
Lanes
1. 1.25 μg/lane
2. 2 μg/lane
3. 3μg/lane
4. 4 μg/lane
The purity of human, recombinant Chitotriosidase expressed in HEK 293 cells (Cat. No. SAE0052) was assessed on SDS-PAGE under reducing conditions (+DTT) followed by InstantBlue™ staining (Cat. No. ISB1L).
(Representative result)
Lanes
1. 1.25 μg/lane
2. 2 μg/lane
3. 3μg/lane
4. 4 μg/lane
| assay | 98% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | 50 kDa |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| specific activity | ≥15,000 units/μg protein |
| storage temp. | −20°C |
| Application: | The enzymatic activity of recombinant human CT is measured by its ability to hydrolyze the substrate 4-Methylumbelliferyl beta -D-N,N’,N”-triacetylchito |
| Biochem/physiol Actions: | CT is currently the most reliable biomarker in Gaucher disease - serum and plasma CT activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase; its increased expression was noted in several lysosomal storage diseases and atherosclerosis. |
| General description: | Chitotriosidase (CT) is a human chitinase with catalytic activity upon the substrate chitin, a polymer of ?-1,4-N-acetylglucosamine |
| Other Notes: | AKLVCYFTNWAQYRQGEARFLPKDL |
| Other Notes: | One unit is defined as the amount of CT required to produce 1 pmol of 4-Methylumbelliferone from 4-Methylumbelliferyl beta -D- N’,N”-triacetylchitotrios |
| Physical form: | Supplied as a lyophilized powder containing phosphate buffered saline |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 98% (SDS-PAGE) |
| activity | specific activity: ≥15,000 units/μg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.14 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |