Peptidyl arginine deiminase type-2 (PAD2) human
SIGMA/SAE0061 - recombinant, expressed in E. coli
Synonym: PAD-2; PAD2; PADI-2; PADI2
Product Type: Chemical
Typical SDS-PAGE analysis of Peptidyl-arginine deiminase, Type 2 (SAE0061)
Activity of PADI2 (SAE0061) was measured at different temperatures. Results are shown as percent of activity measured at 55 °C (unit definition temperature).
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥40 units/mg protein |
| storage temp. | −20°C |
| General description: | Peptidyl Arginine Deiminases (PADs) perform post-translational deiminations of proteins. PADs are calcium-dependent enzymes that catalyze the conversion of Protein--[ This deimination provides another level of regulating protein function. There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity. PADenzymes play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of these enzymes has been found in several diseases such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus, Parkinson′s disease, and cancer. PAD2 is widely expressed in the brain, secretory glands, and skeletal muscles. The enzyme was found to be responsible for hyper-citrullination of myelin basic protein, which is suspected to lead to multiple sclerosis. The product is supplied in 20 mM Tris, pH 7.5, 200 mM NaCl, 10% (w/v) glycerol, 10 mM 2-mercaptoethanol, 1 mM EDTA, 1 mM DTT, 10 mM CaCl2, 10 mM arginine, 30 mM maltose, and 0.6 mg/ml dodecyl−β |
| Physical form: | Supplied as a buffered aqueous solution containing Trizma, NaCl, glycerol, mercaptoethanol, EDTA, DTT, CaCl2, arginine, maltose, and dodecyl-β-D-maltoside. |
| Preparation Note: | Contains a maltose-binding protein fusion tag. |
| Unit Definition: | One unit will produce 1.0 μmole of N-a-benzoylcitrulline ethyl ester from BAEE per hr at 55 °C at pH 7.2. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥40 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.3.15 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352200 |