Angiotensin Converting Enzyme-2, ACE2
SIGMA/SAE0064 - Human recombinant, ≥1000000 U/mg, expressed in HEK 293 cells
Synonym: ACE-related carboxypeptidase; ACE2; Angiotensin-
Product Type: Chemical
The purity of ACE2 Human recombinant (Cat. No. SAE0064) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
Angiotensin II is a systemic vasoconstrictor that causes an increase in blood pressure. Renin, a protease secreted from the kidney, produces angiotensin I by cleaving several amino acids from angiotensinogen. In the next step, angiotensin I is hydrolyzed by ACE (Angiotensin-Converting Enzyme) originating in the lungs, to yield active angiotensin II. Angiotensin II also stimulates the adrenal cortex to secret aldosterione which also causes an increase blood pressure by stimulating sodium reabsorption by the kidney.
ACE2 Human recombinant (Cat. No. SAE0064) was either treated with N glycosidase F (lane 2) or left untreated (lane 1), and separated on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L). Lanes 1. SAE0064 (- N glycosidase F) 2. SAE0064 (+ N glycosidase F)
Angiotensin Converting Enzyme-2 (ACE2) is a membrane-associated and secreted enzyme. SARS-CoV-2 and SARS-CoV-1 use the host ACE2 as a co-receptor to gain intracellular entry into the lungs and brain. The virion expresses a protein termed spike, which directly binds to the extracellular domain of ACE2. A specific region in the spike protein serves as the receptor binding domain (RBD).
The activity of ACE2 Human recombinant (Cat. No. SAE0064) was measured using the fluorogenic substrate Mca-YVADAPK(Dnp)-OH. Shown is the enzymatic activity using different amounts of ACE2.
The effect of increasing amounts of ACE2 Human recombinant (Cat. No. SAE0064) on the enzymatic reaction rate (substrate level is constant).
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg protein Endotoxin |
| mol wt | ~85.9 kDa by SDS-PAGE |
| Quality Level | 100  |
| 200 |
|
| recombinant | expressed in HEK 293 cells |
| shipped in | ambient |
| specific activity | ≥1000000 U/mg |
| storage temp. | −20°C |
| UniProt accession no. | Q9BYF1  |
| Application: | Angiotensin Converting Enzyme-2 (ACE2) has been used in the ACE-2 inhibition assays for inhibitor selectivity studies. It has also been used for identifying potent ACE2-blocking monoclonal antibodies in an on-chip assay and in human-ACE2-blocking assay using a biolayer interferometry biosensor. This approach serves for rational vaccine designing and for the selection of robust immunotherapeutic agents against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. |
| Biochem/physiol Actions: | Recombinant human Angiotensin Converting Enzyme-2 (ACE-2) is expressed in human HEK 293 cells as a C-terminally flag and histidine-tagged glycoprotein with a calculated molecular mass of 85.9 kDa (amino acids Gln18-Ser740, with a C-terminal 10-His tag). The DTT-reduced protein migrates as a 90-120 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. |
| Biochem/physiol Actions: | The angiotensin converting enzyme-2 (ACE-2) functions as carboxydipeptidase. The central role of ACE-2, is to counter ACE activity by reducing the bioavailability of angiotensin (Ang)-II and increasing the Ang(1-7) formation. ACE-2 is a part of the renin-angiotensin system (RAS). Many researches show that Ace-2 ensures the protection of peripheral tissues and might be efficient to treat RAS-related diseases. Also, an imbalance in Ace-2/Ang-(1-7) and ACE/Ang-II axes is important for the onset of cardiovascular diseases. Severe acute respiratory syndrome (SARS) and human coronavirus (HCoV)-NL63 infection viruses has been shown to use its surface protein spike (glycoprotein) to bind to human ACE-2 receptor. The S protein is cleaved into subunits, S1 and S2 during the COVID-19 infection. S1 contains the receptor binding domain (RBD) which enables coronaviruses to bind to the peptidase domain (PD) of ACE2. Thus, ACE2 has become a high focus research target for COVID-19 infection. |
| General description: | The gene for angiotensin converting enzyme-2 (ACE-2) is mapped to human chromosome Xp22.2. This protein is the first known human homologue of ACE. ACE2 has been identified from 5′ sequencing of a human heart failure ventricle cDNA library. It contains a signal peptide, one metalloprotease active site and a transmembrane domain. ACE-2 is a secreted and an integral membrane protein. It is expressed predominantly on the endothelium. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. |
| Unit Definition: | One unit is defined as the amount of enzyme required to cleave 1 picomole of the fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH in one minute, in 37 °C, pH 7.5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| activity | specific activity: ≥1000000 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.17.23 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |