Cholera Toxin B subunit
SIGMA/SAE0069 - recombinant, expressed in HEK 293 cells, subunit A free
Synonym: CTB; CTxB; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Choleragenoid; ToxB
Product Type: Chemical
assay | ≥95% (SDS-PAGE) |
form | lyophilized powder |
mol wt | calculated mol wt 13 kDa |
observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing) | |
pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing) (Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.) | |
quality | (The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.) |
Quality Level | 200 ![]() |
recombinant | expressed in HEK 293 cells |
solubility | H2O: soluble 11 mg/mL |
specific activity | (Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml) |
storage temp. | −20°C |
UniProt accession no. | P01556 ![]() ![]() |
Analysis Note: | Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL. |
Application: | Cholera Toxin B subunit has been used: • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4. • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells. |
General description: | The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124). |
Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. |
Reconstitution: | When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (SDS-PAGE) |
activity | specific activity: (Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |