HIV-1 GP120 protein
SIGMA/SAE0071 - recombinant, expressed in HEK 293 cells
Synonym: Glycoprotein 120 (gp120); Surface protein gp120
Product Type: Chemical
The purity of HIV-1 GP120 protein recombinant (Cat. No. SAE0071) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
HIV-1 gp120 protein is a glycoprotein exposed on the surface of the HIV envelope. The protein′s name is derived from its apparent molecular mass of 120 kDa.1 The gp120 protein is essential for virus entry into cells, as it plays a vital role in attachment to specific cell surface receptors.
| assay | ≥95% (SDS-PAGE) |
| description | low endotoxin |
| form | lyophilized powder |
| impurities | ≤1 EU/μg protein endotoxin |
| mol wt | calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation) |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| UniProt accession no. | P04578  |
| Biochem/physiol Actions: | Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. It was discovered by Tun-Hou Lee and Myron Essex of the Harvard School of Public Health in 1988. The protein′s name is derived from its apparent molecular weight of 120 kDa. The gp120 protein is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and the CD4 receptor. The presence of gp120 is associated with higher levels of plasma IL-6, IL-10, and TNF-α, which may contribute to immune dysfunction during early HIV infection. |
| General description: | Recombinant human glycoprotein 120 (gp120) is expressed in human HEK 293 cells as an C-terminally his-tagged glycoprotein with a calculated molecular mass of 55 kDa (amino acids Lys33-Arg511, with a C-terminal 8-His tag). The DTT-reduced protein migrates as a 100-130 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |