Angiotensin Converting Enzyme, ACE, human
SIGMA/SAE0075 - recombinant, ≥10 U/mg, expressed in HEK 293 cells
Synonym: ACE; ACE1; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; Peptidyl-dipeptidase A; Peptidyldipeptide Hydrolase
Product Type: Chemical
Angiotensin II is a systemic vasoconstrictor that causes an increase in blood pressure. Renin, a protease secreted from the kidney, produces angiotensin I by cleaving several amino acids from angiotensinogen. In the next step, angiotensin I is hydrolyzed by ACE (Angiotensin-Converting Enzyme) originating in the lungs, to yield active angiotensin II. Angiotensin II also stimulates the adrenal cortex to secret aldosterione which also causes an increase blood pressure by stimulating sodium reabsorption by the kidney.
The purity of Angiotensin Converting Enzyme, ACE, human recombinant (Cat. No. SAE0075) was assessed on SDS-PAGE stained with InstantBlue™ (Cat. No. ISB1L).
The activity of Angiotensin Converting Enzyme, ACE, human recombinant was measured using the ACE-1 fluorogenic substrate, MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-DNP-Lys (Cat. No. SCP0131). Lanes 1. ACE1 (Cat. No. SAE0075) 2. Competitor R
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation) |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| specific activity | ≥10 U/mg |
| storage temp. | −20°C |
| UniProt accession no. | P12821  |
| Biochem/physiol Actions: | Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy. |
| General description: | Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags. |
| General description: | The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. |
| Specificity: | The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU |
| Unit Definition: | One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| activity | specific activity: ≥10 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.15.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |