MMP-9 pre-activated human
SIGMA/SAE0078 - recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells
Synonym: GELB; GELBCLG4B; Gelatinase; Gelatinase B; MANDP2; MMP-9; Matrix Metalloproteinase-10; Type IV collagenase
Product Type: Chemical
The activity of MMP-9 pre-activated human recombinant (Cat. No. SAE0078) was measured using the MMMP-9 fluorogenic substrate, MCA-Lys-Pro-Leu-Gly-Leu-DNP-Dpa-Ala-Arg-NH2 (Cat. No. SCP0193).
| application(s) | cell analysis |
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| concentration | 30-120 μg/mL (50 μg protein, determined by Bradford. The protein concentration is 30-120 μg/ml. for the lot-specific concentration, see Certificate of Analysis.) |
| form | liquid |
| mol wt | calculated mol wt 66 kDa |
| observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation) | |
| Quality Level | 200  |
| recombinant | expressed in HEK 293 cells |
| shipped in | dry ice |
| storage temp. | −20°C |
| UniProt accession no. | P14780  |
| Biochem/physiol Actions: | MMP-9 is a member of the matrix metalloproteinase (MMP) family of proteins. Proteins of the MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8 (interleukin-8)-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of extracellular matrix (ECM) remodeling. MMP-9 degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target. As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. MMP-9 can be cleaved (and thus activated) in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. Structurally, MMP9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline rich linker region, and a carboxyl terminal hemopexin like domain. As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. This product was cleaved and activated in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563. |
| General description: | Recombinant human pre-activated Matrix Metalloproteinase-9 (MMP-9) is expressed in human HEK 293 cells as a glycoprotein. The DTT-reduced protein migrates as a ~67 kDa polypeptide on SDS-PAGE. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags. |
| Other Notes: | This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation. |
| Physical form: | Liquid solution, 0.22 mm filtered, containing 25 mM Tris, 10 mM CaCl2, 0.05% Brij-35 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.24.35 ( BRENDA | IUBMB ) |
| UNSPSC | 12352200 |