Auresine
SIGMA/SAE0083 - recombinant, lyophilized powder
Product Type: Chemical
| form | lyophilized powder |
| mol wt | 14.4 kDa |
| recombinant | expressed in Pichia pastoris |
| specific activity | >500 units/mg protein |
| storage condition | room temperature (at +4 °C (in Storage Buffer)) |
| storage temp. | 2-8°C |
| Application: | Auresine can be used for isolation of protoplasts, nucleic acids, proteins, lipids and other components of Gram-positive bacterial cells. Auresine is particularly useful to lyse MRSA.. Thanks to its unique activity in non-physiological conditions (low conductivity and low temperatures), isolated components after auresine treatment, like nucleic acids and proteins, are not prone to degradation by released cellular enzymes. |
| General description: | Auresine is a portion of the zinc metalloprotease Staphylococcus aureus. The enzyme has glycyl-glycine endopeptidase activity and it specifically cleaves polyglycine crosslinks in the cellular wall of Staphylococcus species, including various strains of methicillin-resistant Staphylococcus aureus (MRSA). Auresine is thus useful for lysis of MRSA. |
| Preparation Note: | Auresine in soluble in water (20 mg/ml), yielding a clear solution. The product is active in 100 nM concentration (1.4 ug/mL) in low conductivity buffers (= 2 mS/cm) at temp. 0-40°C. |
| Storage and Stability: | Auresine lyophilized powder should be stored at room temperature. Once dissolved in storage buffer, Auresine is stable at room temperature up to 4 months, at 4°C, -20°C, -80°C at least 2 years. Storage buffer: 20 mM Tris-HCl, pH 7.0, 200 mM NaCl, 10% glycerol. |
| Unit Definition: | One unit will reduce the turbidity (A600) of suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes in 50 mM glycine, pH 8.0 at 25°C in 6.0 mL reaction mixture. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >500 units/mg protein |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |