IDO1, human
SIGMA/SAE0096 - recombinant, expressed in E. coli, lyophilized powder, His tagged
Synonym: Indoleamine 2,3-dioxygenase 1
Product Type: Chemical
SDS-PAGE IDO1, human (Cat. No. SAE0096) was assessed on SDS-PAGE.
| form | lyophilized powder |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | 800 units/μg |
| storage temp. | −20°C |
| UniProt accession no. | P14902   |
| General description: | IDO1 is a heme-enzyme that is induced by INF-γ and catalyzes the conversion of tryptophan to kynurenine. This leads to a depletion of tryptophan that results in T cells and dendritic cells inactivation. IDO activity has been identified as an important immune effector pathway in tumor cells to escape a potentially effective immune response. Inhibition of IDO1 has been found to delay cancer growth and enhance dendritic vaccines. Therapeutic inhibition of IDO1 is of interest because of its proposed role in the pathogenesis of several diseases, including cancer, hypotension, neurodegenerative disorders, and its potential to aid cancer immunotherapy and organ transplant. |
| General description: | This recombinant human IDO1 has a 6x histidine tag at its N-terminus. This product is lyophilized from a solution containing 10 mM Trizma buffer (pH 7.0), with 500 mM NaCl and a carbohydrate carrier. |
| Unit Definition: | One unit of enzyme hydrolyzes 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH-6.5 at 37°C |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 800 units/μg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.13.11.52 ( BRENDA | IUBMB ) |
| UNSPSC | 12352202 |