D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans
SIGMA/SAE0097 - recombinant, expressed in E. coli, aqueous solution
Synonym: D2HGDH; HGDH; L-2-hydroxyglutarate dehydrogenase
Product Type: Chemical
| assay | ≥95% (SDS-PAGE) |
| form | aqueous solution |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | ≥1000 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | D2RJU7   |
| General description: | D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD+ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD+ . The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as: • A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate. • Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates. • A coupled assay system to measure branched-chain amino acid aminotransferase activity. |
| Legal Information: | T3P is a registered trademark of Archimica GmbH |
| Preparation Note: | This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol. |
| Unit Definition: | One unit of enzyme oxidizes 1 μmole of NADH to NAD+ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| activity | specific activity: ≥1000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1. 99.2 |
| UNSPSC | 12352202 |