Lyticase from Arthrobacter luteus
SIGMA/SAE0098 - free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Synonym: (1,3)-β-D-Glucan endohydrolase; 1,3-β-Glucan glucohydrolase
CAS Number: 37340-57-1
MDL Number: MFCD00131560
Product Type: Chemical
Lyticase specificity: Yeast β-glucan is a polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.
PCR Amplification of 16S rDNA Using Universal Primer Set PCR amplification was conducted using 16S universal primers to amplify a 1,500 bp amplicon. Lanes M: 1 kb ladder (D3937) 1: positive control containing only genomic DNA from Proteus mirabilis 2: negative control with water only 3: heat inactivated Lyticase 4: Lyticase spiked with genomic DNA Heat inactivated Lyticase is shown to be free of DNA contaminants by lack of amplified 16S amplicon in reaction containing only sample (Lane 3), compared to successful amplification of the 16S amplicon in reaction spiked with genomic DNA (Lane 4).
| biological source | bacterial (Arthrobacter luteus) |
| feature | DNA free |
| form | lyophilized powder |
| shipped in | dry ice |
| specific activity | ≥2000 units/mg protein |
| storage temp. | −20°C |
| Biochem/physiol Actions: | Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan. |
| General description: | Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species. Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis  at www.sigma-aldrich.com/enz |
| Unit Definition: | One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2000 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |