Lyticase from Arthrobacter luteus
SIGMA/SAE0098 - free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder
Synonym: (1,3)-β-D-Glucan endohydrolase; 1,3-β-Glucan glucohydrolase
CAS Number: 37340-57-1
MDL Number: MFCD00131560
Product Type: Chemical
biological source | bacterial (Arthrobacter luteus) |
feature | DNA free |
form | lyophilized powder |
shipped in | dry ice |
specific activity | ≥2000 units/mg protein |
storage temp. | −20°C |
Biochem/physiol Actions: | Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan. |
General description: | Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species. Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets. |
Other Notes: | View more information on enzymes for complex carbohydrate analysis ![]() ![]() |
Unit Definition: | One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H334 |
Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
activity | specific activity: ≥2000 units/mg protein |
Storage Temp. | −20°C |
UNSPSC | 12352202 |